Paper Chromatographic Separation of Barbituric Acids

Separation of Amino Acids by Paper Chromatography

Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

Chromatographic Separation, and Characteristics of Nucleic Acids from HeLa Cells

The application of the phenol-duponol method to extraction of nucleic acids from HeLa cells is described. Chromatography of the phenol extract on an esterified bovine serum albumin column with a salt gradient of sodium chloride gives separation of soluble RNA, DNA, and two different high molecular RNA fractions. Ultracentrifugation of the DNA eluted from the column gives a sedimentation coeffic...

Chromatographic separation of mononucleotides derived from transfer ribonucleic acids.

1. A method is described for fractionating alkaline and enzymic hydrolysates of ribonucleic acids and oligonucleotides on DEAE-cellulose columns by using a constant-composition eluent. 2. The relative partition coefficients of the four major and several minor nucleotides present in nucleic acid digests are given. 3. The influence of pH and of molarity of the buffer on the separation is describe...

Two-dimensional paper chromatographic determination of amino acids in urine.

DENT (1) introduced paper chromatography for tile ilivestigatioll of amino-aciduria in 1948. However, his one-dimensional system yielded inadequate separation, and his two-dimensional method required 3 days. Since then, technics utilizing gas chromatography (2-4), thin layer chromatography, column chromatography, and high voltage electrophoresis (5-7) have been published for tile determination ...

Chromatographic Separation and Identification of Organic Acids Application to Detection of Organic Acids in River Waters